Borosil Pricelist 2021-22

Autoclaves are widely used to sterilise instruments, glassware and plasticware, solutions and media, and to decontaminate biological wastes. Due to the physical hazards (e.g. heat, steam and pressure) associated with autoclaving, extra care must be taken to use them safely . Each autoclave has unique characteristics and operating requir ements. Please review and under stand the owner's manual before using any autoclave for the first time, and every time thereafter . Also, please follow all the safety regulations for autoclaving, laid down by your institution. 1. Place individual glassware vessels within a heat r esistant plastic or metal tray on a shelf or rack. 2. Never place them directly on the autoclave base or floor 3. Always maintain space (>5 cm) between glass vessels to give them r oom to expand while heating, and to prevent them fr om hitting other vessels when entering or leaving the autoclave 4. Add water rising up to 1/4 to 1/2 inch in the tray , so that the glassware will heat mor e evenly 5. Large heavy glasswar e, such as 3L or larger spinner flasks 5L or larger bottles and carboys should have a small amount of distilled water placed inside them to help generate steam when they are being sterilised dry in an autoclave Autoclaving empty glassware Safety during Autoclaving Always follow the safety precautions to protect yourself and the equipment being used. Autoclaving glassware containing liquids Never autoclave items containing corrosives (e.g. acids, bases, phenol) , solvents or volatiles (e . g . ethanol , m ethanol , chloro form) or radioactive materials. 1. Fill the glassware only half full with the liquids to be sterilised. 2. Take into consideration, the volume of liquid to be autoclaved. For e.g. : 2-litre flask containing 1 litre of liquid takes much longer to sterilise than four 500 ml flasks, each containing 250 ml of liquid 3. Do a test run when autoclaving larger volumes of liquid, to make sure that the liquid has reached 121ºC 4. Always use the slow exhaust or liquid cool cycle when autoclaving liquids, to prevent the liquid fr om boiling over , causing loss of the contents 5. Suspend an autoclave thermometer with a thin wire, in the middle of the liquid-filled container to record the highest temperatur e r eached. Then, check its reading after the autoclave run is complete. If the temperatur e is too low , then the run-time will need to be increased or the volume will need to be reduce 6. To prevent bottles fr om getting shattered during pr essurisation, the caps of the vessels with liquids must be loosened completely before loading 1. Be sure to wear the corr ect PPE including a lab coat, eye pr otection, closed-toe shoes, and heat-r 2. Remember that after usage, glassware will be hot and can cause serious injury if the correct precautionary measur es aren't taken 3. Always wait for the pressure gauge to drop to zero with zero time r emaining before you open the door 4. Stand behind the door as you open it and allow all the steam to fully escape before reaching into the autoclave 5. Let glassware fully cool before touching them DO'S Do not tighten caps on the vessels immediately af ter autoclaving The cap or the vessel may get shatter ed due to the vacuum r esulting fr om the cooling of the steam in the vessel DON'T’S W ait for 5 minutes befor e r emoving dr y glassware fr om the autoclave, and wait for 10 to 20 minutes befor e r emoving glassware containing liquids Do not put hot glasswar e, especially large bottles, from an autoclave (or any other heat source) on to a cold bench top. The str ess that is induced will often cause the base of the vessel to crack or fall o ff, r eleasing its hot contents For non-liquid loads, please allow the glasswar e to cool for 15 minutes befor e touching it For liquid loads, please allow the liquid to stand for a full hour before touching the glassware AUTOCLAVING OF GLASSWARE esistant gloves